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Ultrafiltration and Diafiltration
Author: Rachel C. Houp

“Biotech Processes” discusses fundamental information about biotechnology manufacturing useful to practitioners in validation and compliance. Reader comments, questions, and suggestions are needed to make this column a useful resource for daily work applications. The key objective for this column: useful information.

Contact column coordinator David Fetterolf at dfetterolf@biotechlogic.com or journal coordinating editor Susan Haigney at shaigney@advanstar.com with comments, questions, or suggestions for future discussion topics.

KEY POINTS
The following key points are discussed in this article:
• Ultrafiltration (UF) is a commonly used biotech process for concentrating a dilute product stream. UF separates molecules in solution based on the membrane pore size or molecular weight cutoff.
• Diafiltration (DF) is most often used to exchange product into a desired buffer (e.g., from an elution buffer into a final formulation buffer)
• UF and DF typically use tangential flow filtration, where feed flows parallel to the membrane surface rather than perpendicular to the surface.

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